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KMID : 0380620180500040383
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Korean Journal of Food Science and Technology
2018 Volume.50 No. 4 p.383 ~ p.390
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Neuroprotective effects of cultured and fermented wild ginseng extracts on oxidative stress induced by hydrogen peroxide in PC12 cells
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Choi Yeo-Ok
Kim Yu-Ri
Shin Seung-Yong
Lee Jae-Geun
Kim Chul-Joong
Lee Ye-Ji
Kang Byeong-Ju
Kim Gwan-Su
Choi Jee-Eun
Han Beom-Seok
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Abstract
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Most neurodegenerative diseases are known to be influenced by oxidative stress. We investigated the antioxidative activity of the concentrate of fermented wild ginseng root culture (HLJG0701) containing ginsenosides Rg5 and Rk1. HLJG0701 showed effective DPPH and ABTS radical scavenging ability (IC50: 16- and 4-fold dilution, respectively) and was inhibited dose-dependently by the FeSO4-induced lipid peroxidation group (8- and 4-fold dilution: 2.3 and 1.5 nM, respectively). In MTT and LDH assays, 8-, 16-, 32- and 64-fold diluted HLJG0701 significantly increased cell viability by 70, 53, 35, and 26%, respectively. LDH released by HLJG0701 was reduced 1.3-fold with 8-fold diluted HLJG0701 compared to the H2O2-treated control. In addition, the inhibitory effect of HLJG0701 on oxidative stress in PC12 cells was confirmed by DCF-DA analysis (16-, 4-fold diluted HLJG0701: 50 and 68% ROS inhibition, respectively), TBARS (16- and 4-fold diluted HLJG0701: 50.7 and 46.5% inhibition, respectively), GPx (16- and 4-fold diluted HLJG0701: 133.3 and 227.3% release, respectively), and SOD analysis (16- and 4-fold diluted HLJG0701: 118.2 and 218.2% release, respectively). These results suggested that HLJG0701 protects neuronal cells by its anti-oxidative effects and hence can be a potential preventive material against neurodegenerative diseases.
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KEYWORD
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cultivated wild ginseng, oxidative stress, neurodegenerative diseases, ginsenoside Rg5, ginsenoside Rk1, PC12 cell
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